Comparative genomics of transcriptional regulation in yeast and its application to identification of a candidate alpha-isopropylmalate transporter

Galina Yu Kovaleva, Georgii A. Bazykin, Michael Brudno, Mikhail S. Gelfand

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Conservation rates in non-protein-coding regions of five yeast genomes of the genus Saccharomyces were analyzed using multiple whole-genome alignments. This analysis confirmed previously shown decrease in conservation rates observed immediately upstream of the translation start point and downstream of the stop-codon. Further, there was a sharp conservation peak in the upstream regions likely related to the core promoter (-35 bp to +35 bp around TSS) and a conservation peak downstream of the stop-codon whose function is not yet clear. Regulation of leucine and methionine biosynthesis controlled by the global regulator Gcn4p and pathway-specific regulators was analyzed in detail. A candidate alpha-isopropylmalate carrier, YOR271cp, was identified based on conservation of Leu3p binding sites, analysis of ChIP-chip data, protein localization and sequence similarity.

Original languageEnglish
Pages (from-to)981-998
Number of pages18
JournalJournal of Bioinformatics and Computational Biology
Volume4
Issue number5
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Keywords

  • Alpha-isopropylmalate transporter
  • Evolution of regulatory signals
  • Regulation of transcription

Fingerprint

Dive into the research topics of 'Comparative genomics of transcriptional regulation in yeast and its application to identification of a candidate alpha-isopropylmalate transporter'. Together they form a unique fingerprint.

Cite this