Comparative analysis of the lipid composition of peritoneal fluid and blood plasma in patients with external genital endometriosis and uterine myoma

Anna Valerevna Borisova, Vitaliy Viktorovich Chagovets, Andrey Vladimirovich Kozachenko, Nataliia Leonidovna Starodubtseva, Alexey Sergeevich Kononikhin, Dinara Failevna Salimova, Eugenia Aleksandrovna Kogan, Leila Vladimirovna Adamyan, Vladimir Evgenievich Frankevich, G. T. Sukhikh

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Objective. To enhance the efficiency of diagnosing external genital endometriosis by direct mass spectrometry. Subjects and methods. A case-control study covered 100 patients with external genital endometriosis (a study group) and 50 patients with uterine myoma (a control group) who had been operated on at the Gynecology Department, V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation. The diagnosis of external genital endometriosis was based on diagnostic and therapeutic laparoscopy and finally confirmed histologically. To determine the lipid composition of blood and peritoneal fluid, their samples were obtained from all the study participants. The lipidome was qualitatively and quantitatively assessed using electrospray mass spectrometry. An analysis revealed more than 140 molecular characteristics, most of which were five lipid classes: phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, diglycerides, and triglycerides. The obtained experimental data were analyzed using the multivariate partial lest squares-discriminant analysis (PLS-DA). The created statistical models well group data based on a case-control sign and could identify lipids, which make the greatest contribution to the clustering of the data. Results. Unlike the comparison group, the patients with endometriosis showed a substantial decrease in the blood and peritoneal fluid level of four lipids: phosphatidylcholine PC 36:4, lysophosphatidylcholine LPC 16:0, sphingomyelin SM 34:1, and phosphoethanolamine PE O-34:1. The level of phosphoethanolamine PE O-20:0 was increased not only in the endometrial tissues, but also in the biological fluids of patients with endometriosis, suggesting the possible diagnostic value of this lipid. The sensitivity and specificity of the method was 93% and 95%, respectively for blood plasma; those were 90 and 95% for peritoneal fluid. Conclusion. This investigation confirms the involvement of phospholipids and sphingolipids in the pathophysiology of endometriosis and opens up new possibilities for the noninvasive diagnosis of external genital endometriosis.

Original languageEnglish
Pages (from-to)74-82
Number of pages9
JournalAkusherstvo i Ginekologiya (Russian Federation)
Issue number6
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • Biomarkers
  • Endometriosis
  • Lysophosphatidylcholine
  • Mass spectrometry
  • Phosphatidylcholine
  • Phosphatidylethanolamine
  • Sphingomyelin

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