Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection

Olga A. Averina, Oleg A. Permyakov, Olga O. Grigorieva, Alexey S. Starshin, Alexander M. Mazur, Egor B. Prokhortchouk, Olga A. Dontsova, Petr V. Sergiev

Research output: Contribution to journalArticlepeer-review

Abstract

Genome editing is an indispensable tool for functional genomics. The caveat of the ge-nome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA tem-plate following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.

Original languageEnglish
Article number10221
JournalInternational Journal of Molecular Sciences
Volume22
Issue number19
DOIs
Publication statusPublished - 1 Oct 2021

Keywords

  • CRISPR Cas9
  • Genome editing
  • Homologous recombina-tion
  • Knockout mice
  • Microinjection
  • Prime editor

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