Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells

Almudena Muñoz Javier, Oliver Kreft, Alicia Piera Alberola, Christian Kirchner, Bernd Zebli, Andrei S. Susha, Elias Horn, Susanne Kempter, Andre G. Skirtach, Andrey L. Rogach, Joachim Rädler, Gleb B. Sukhorukov, Martin Benoit, Wolfgang J. Parak

Research output: Contribution to journalArticlepeer-review

116 Citations (Scopus)

Abstract

We propose a combination of atomic force microscopy (AFM) and optical microscopy for the investigation of particle uptake by cells. Positively and negatively charged polymer microcapsules were chosen as model particles, because their interaction with cells had already been investigated in detail. A FM measurements allowed the recording of adhesion forces on a single-molecule level. Due to the micrometer size of the capsules, the number of ingested capsules could be counted by optical microscopy. The combination of both methods allowed combined measurement of the adhesion forces and the uptake rate for the same model particle. As a demonstration of this system, the correlation between the adhesion of positively or negatively charged polymer microcapsules onto cell surfaces and the uptake of these microcapsules by cells has been investigated for several cell lines. As is to be expected, we find a correlation between both processes, which is in agreement with adsorption-dependent uptake of the polymer microcapsules by cells.

Original languageEnglish
Pages (from-to)394-400
Number of pages7
JournalSmall
Volume2
Issue number3
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Keywords

  • Adsorption
  • Cells
  • Cellular uptake
  • Imaging
  • Microcapsules

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