Combination of low-temperature electrosurgical unit and extractive electrospray ionization mass spectrometry for molecular profiling and classification of tissues

Gennady Sukhikh, Vitaliy Chagovets, Xinchen Wang, Valeriy Rodionov, Vlada Kometova, Alisa Tokareva, Alexey Kononikhin, Natalia Starodubtseva, Konstantin Chingin, Huanwen Chen, Vladimir Frankevich

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue composition at a particular location by surgical smoke analysis is now of increasing interest for clinical use. However, molecular analysis of surgical smoke is commonly lacking molecular specificity and is associated with significant carbonization and chemical contamination, which are mainly related to the high temperature of smoke at which many molecules become unstable. Unlike traditional electrosurgical tools, low-temperature electrosurgical units allow tissue dissection without substantial heating. Here, we show that low-temperature electrosurgical units can be used for desorption of molecules from biological tissues without thermal degradation. The use of extractive electrospray ionization technique for the ionization of desorbed molecules allowed us to obtain mass spectra of healthy and pathological tissues with high degree of differentiation. Overall, the data indicate that the described approach has potential for intraoperative use.

Original languageEnglish
Article number2957
JournalMolecules
Volume24
Issue number16
DOIs
Publication statusPublished - 15 Aug 2019
Externally publishedYes

Keywords

  • Breast cancer
  • EESI
  • Intraoperative navigation
  • Mass spectrometry
  • Molecular imaging

Fingerprint

Dive into the research topics of 'Combination of low-temperature electrosurgical unit and extractive electrospray ionization mass spectrometry for molecular profiling and classification of tissues'. Together they form a unique fingerprint.

Cite this