Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring

Lyubov I. Kazakova, Lyudmila I. Shabarchina, Gleb B. Sukhorukov

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

Enzyme based micron sized sensing system with optical readout was fabricated by co-encapsulation of urease and dextran couple with pH sensitive dye SNARF-1 into polyelectrolyte multilayer capsules. Co-precipitation of calcium carbonate, urease and dextran followed up by multilayer film coating and Ca-extracting by EDTA resulted in the formation of 3.5-4 micron capsules, what enable the calibrated fluorescence response to urea in concentration range from 10-6 to 10-1 M. The presence of urea can be monitored on a single capsule level as illustrated by confocal fluorescent microscopy. Variations in urease:dye ratio in capsules, applicability and limits of use of that type multi-component microencapsulated sensors are discussed.

Original languageEnglish
Pages (from-to)11110-11117
Number of pages8
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number23
DOIs
Publication statusPublished - 21 Jun 2011
Externally publishedYes

Fingerprint

Dive into the research topics of 'Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring'. Together they form a unique fingerprint.

Cite this