Circular Permutants of BrUSLEE Protein as Fluorescent pH Indicators

A. V. Mamontova, T. R. Simonyan, K. A. Lukyanov, A. M. Bogdanov

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract—: Two variants of circularly permuted BrUSLEE, a green fluorescent protein with a short fluorescence lifetime, have been engineered. We characterized the pH-dependence of fluorescence decay kinetics of these fluorophores. It was shown that both permutants (cpBrUS and cpBrUS-145) exhibit three-component fluorescence decay kinetics, with the lifetime of the one component varying within the ~3000–300 ps range upon pH shift from 5.5 to 9.0. At the same time, the original BrUSLEE does not show a significant change in the fluorescence decay kinetics within the physiologically relevant pH-range of 6.0–8.5. The described pH-dependence allows considering the BrUSLEE permutants as pH indicators with the fluorescence lifetime readout.

Original languageEnglish
Pages (from-to)850-853
Number of pages4
JournalRussian Journal of Bioorganic Chemistry
Volume48
Issue number4
DOIs
Publication statusPublished - Aug 2022

Keywords

  • fluorescence lifetime
  • fluorescent indicators
  • fluorescent proteins
  • GFP

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