Chemodiagnostic by lipid analysis of the microbial community structure in trace metal polluted urban soil

V. A. Terekhova, N. V. Verkhovtseva, M. A. Pukalchik, I. R. Vodolazov, V. K. Shitikov

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

This study is focused on assessment of microbial community structure differences in urban soils by means of lipid analysis. Soil samples (0–20 cm deep topsoil layer) contaminated with trace metals to various extents were taken from six sites located in the rural part of the city of Kirov (Russia). The samples were measured for pH and total Pb, Ni, Cr, and Cd. To study the microbial community, microorganism markers (fatty acids) were extracted, which were then diagnosed by mass spectrometry. Nonmetric multidimensional scaling plots of soil communities showed relative dissimilarity in control and polluted soils. The total lipid content in control samples was found to be significantly higher than in polluted soil. The highest indicator value was assigned to Actinobacteria phylum, whose concentration decreased remarkably in polluted samples, and anaerobes Butyrivibrio sp. and Bifidobacterium sp. were regarded as an indicator for soils with relatively low pollution exposure. Microbial profiles were also indicative of selective enrichment with competent species (Desulfovibrio sp., Bacteroides fragilis, and Chlamydia sp.) in soil greatly contaminated with heavy metals. This study suggests that the method of lipid diagnostic can be highly indicative of soil microbial structure and thus it can be used as a quantitative measurement of urban soil biological quality.

Original languageEnglish
Title of host publicationSpringer Geography
PublisherSpringer
Pages150-160
Number of pages11
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameSpringer Geography
ISSN (Print)2194-315X
ISSN (Electronic)2194-3168

Keywords

  • Heavy metal
  • Lipid markers
  • Microbial biodiversity
  • Nonmetric multidimensional scaling
  • Ordination
  • Soil quality
  • Urban soil

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