Characterization of the proteomic profile of cosmonauts' urine following long-duration space missions using a semiquantitative approach

A. G. Brzhozovsky, A. S. Kononikhin, M. I. Indeikina, D. S. Kashirina, I. A. Popov, L. Kh Pastushkova, E. N. Nikolaev, I. M. Larina

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Effects of space flight (SF) factors on cosmonaut's organism were studied using the urine proteome analysis. Urine samples were collected 6 months before launch and on days 1 and 7 of recovery (R). Urine proteins were subjected to enzymatic hydrolysis and the resulted mix of peptides was identified with tandem chromatography-mass spectrometry. Identified were proteins significantly changed after SF. The data were used for clustering proteins in order to establish their interactions. Out of 256 proteins 50 were typical of this sample including 20 proteins that were changed significantly on R +1. Increase in their levels can be a consequence of the descent module landing (SF final stage) or deadaptation in the Earth's gravity. Also, mean values of protein concentrations were found to be similar to the values measured on R + 1 implying uniformity of changes due to SF factors affecting human organism. Analysis of proteomic changes in cosmonauts who have flown repeatedly points to protein profile modification between missions.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalAviakosmicheskaya i Ekologicheskaya Meditsina
Volume51
Issue number6
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • Cosmonaut
  • Mass-spectrometry
  • Proteomics
  • Space flight
  • Urine proteome

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