Changes in spectral properties and composition of lipofuscin fluorophores from human-retinal-pigment epithelium with age and pathology

Tatiana B. Feldman, Marina A. Yakovleva, Patimat M. Arbukhanova, Sergey A. Borzenok, Alexey S. Kononikhin, Igor A. Popov, Evgeny N. Nikolaev, Mikhail A. Ostrovsky

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Fundus autofluorescence mostly originates from bisretinoid fluorophores in lipofuscin granules, which accumulate in retinal-pigment-epithelium cells with age. The dynamics of accumulation, photo-oxidation, and photodegradation of bisretinoids during aging or in the presence of pathology have been insufficiently investigated. Changes in spectral properties and composition of human lipofuscin-granule fluorophores with age and pathology have now been investigated by a high-performance liquid chromatography method using spectrophotometric and fluorescent detectors connected in series. It was found that: (i) N-retinylidene-N-retinylethanolamine (A2E) fluorescence intensity is not predominant in the chloroform extract of human-cadaver-eye retinal pigment epithelium studied; bisretinoid photo-oxidation and photodegradation products have much higher fluorescent properties; (ii) the relative emission maximum in the fluorescence spectrum of suspended retinal-pigment-epithelium cells obtained from an individual human-cadaver eye without pathology is irrespective of donor age and falls within the range 575±15 nm; in two cadaver eyes with signs of age-related macular degeneration, emission maxima were shifted by 23-36 nm towards the shortwave region; and (iii) the ratio of bisretinoid photo-oxidation and photodegradation products to unoxidized bisretinoids in the chloroform extract of cadaver-eye retinal pigment epithelium increases with donor age, from 0.69±0.03 to 1.32±0.04. The differences in fluorescence properties between chloroform extracts obtained from cadaver eyes with and without signs of age-related macular degeneration could be used to increase the potential of fundus autofluorescence imaging as a noninvasive diagnostic method.

Original languageEnglish
Pages (from-to)1075-1088
Number of pages14
JournalAnalytical and Bioanalytical Chemistry
Volume407
Issue number4
DOIs
Publication statusPublished - 24 Jan 2015
Externally publishedYes

Keywords

  • Bisretinoid fluorophores
  • Fundus autofluorescence
  • High-performance liquid chromatography
  • Lipofuscin granule
  • Retinal pigment epithelium

Fingerprint

Dive into the research topics of 'Changes in spectral properties and composition of lipofuscin fluorophores from human-retinal-pigment epithelium with age and pathology'. Together they form a unique fingerprint.

Cite this