Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Ekaterina M. Merzlyak, Joachim Goedhart, Dmitry Shcherbo, Mariya E. Bulina, Aleksandr S. Shcheglov, Arkady F. Fradkov, Anna Gaintzeva, Konstantin A. Lukyanov, Sergey Lukyanov, Theodorus W.J. Gadella, Dmitriy M. Chudakov

Research output: Contribution to journalArticlepeer-review

470 Citations (Scopus)

Abstract

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

Original languageEnglish
Pages (from-to)555-557
Number of pages3
JournalNature Methods
Volume4
Issue number7
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

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