Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova, Jamal Alzubi, Valentina Pennucci, Marie Follo, Boris Kochergin, Albert Muslimov, Ilya Skovorodkin, Seppo Vainio, Maria N. Antipina, Vsevolod Atkin, Alexey Popov, Igor Meglinski, Toni Cathomen, Tatjana I. Cornu, Dmitry A. Gorin, Gleb B. Sukhorukov, Irina Nazarenko

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

    Original languageEnglish
    Article number1904880
    JournalSmall
    Volume16
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 2020

    Keywords

    • biomimetics
    • drug delivery
    • extracellular vesicles
    • gene delivery
    • microcapsules
    • nanocapsules

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