Barnase encapsulation into submicron porous CaCO3particles: Studies of loading and enzyme activity

Alexey M. Yashchenok, Olga I. Gusliakova, Elena V. Konovalova, Marina V. Novoselova, Victoria O. Shipunova, Tatiana O. Abakumova, Olga I. Efimova, Roman Kholodenko, Alexey A. Schulga, Timofei S. Zatsepin, Dmitry A. Gorin, Sergey M. Deyev

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The present study focuses on the immobilization of the bacterial ribonuclease barnase (Bn) into submicron porous calcium carbonate (CaCO3) particles. For encapsulation, we apply adsorption, freezing-induced loading and co-precipitation methods and study the effects of adsorption time, enzyme concentration and anionic polyelectrolytes on the encapsulation efficiency of Bn. We show that the use of negatively charged dextran sulfate (DS) and ribonucleic acid from yeast (RNA) increases the loading capacity (LC) of the enzyme on CaCO3 particles by about 3-fold as compared to the particles with Bn itself. The ribonuclease (RNase) activity of encapsulated enzyme depends on the LC of the particles and transformation of metastable vaterite to stable calcite, as studied by the assessment of enzyme activities in particles. This journal is

Original languageEnglish
Pages (from-to)8823-8831
Number of pages9
JournalJournal of Materials Chemistry B
Volume9
Issue number42
DOIs
Publication statusPublished - 14 Nov 2021

Fingerprint

Dive into the research topics of 'Barnase encapsulation into submicron porous CaCO3particles: Studies of loading and enzyme activity'. Together they form a unique fingerprint.

Cite this