Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors

Ilya A. Osterman, Irina V. Prokhorova, Vasily O. Sysoev, Yulia V. Boykova, Olga V. Efremenkova, Maxim S. Svetlov, Vyacheslav A. Kolb, Alexey A. Bogdanov, Petr V. Sergiev, Olga A. Dontsova

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.

Original languageEnglish
Pages (from-to)1774-1783
Number of pages10
JournalAntimicrobial Agents and Chemotherapy
Volume56
Issue number4
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

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