Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic microcin C

Anastasia Metlitskaya, Teymur Kazakov, Aigar Kommer, Olga Pavlova, Mette Praetorius-Ibba, Michael Ibba, Igor Krasheninnikov, Vyacheslav Kolb, Inessa Khmel, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

115 Citations (Scopus)

Abstract

Microcin C is a ribosome-synthesized heptapeptide that contains a modified adenosine monophosphate covalently attached to the C-terminal aspartate. Microcin C is a potent inhibitor of bacterial cell growth. Based on the in vivo kinetics of inhibition of macromolecular synthesis, Microcin C targets translation, through a mechanism that remained undefined. Here, we show that Microcin C is a subject of specific degradation inside the sensitive cell. The product of degradation, a modified aspartyl-adenylate containing an N-acylphosphoramidate linkage, strongly inhibits translation by blocking the function of aspartyl-tRNA synthetase.

Original languageEnglish
Pages (from-to)18033-18042
Number of pages10
JournalJournal of Biological Chemistry
Volume281
Issue number26
DOIs
Publication statusPublished - 30 Jun 2006
Externally publishedYes

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