Application of spiroplasma melliferum proteogenomic profiling for the discovery of virulence factors and pathogenicity mechanisms in host-associated spiroplasmas

Dmitry Alexeev, Elena Kostrjukova, Alexander Aliper, Anna Popenko, Nikolay Bazaleev, Alexander Tyakht, Oksana Selezneva, Tatyana Akopian, Elena Prichodko, Ilya Kondratov, Mikhail Chukin, Irina Demina, Maria Galyamina, Dmitri Kamashev, Anna Vanyushkina, Valentina Ladygina, Sergei Levitskii, Vasily Lazarev, Vadim Govorun

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.

Original languageEnglish
Pages (from-to)224-236
Number of pages13
JournalJournal of Proteome Research
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Jan 2012
Externally publishedYes

Keywords

  • metabolomics
  • proteogenomics
  • Spiroplasma
  • systems approach
  • virulence factors

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