Application of deuterium–hydrogen exchange to study the secondary structure of oligonucleotide ions in a gas phase

Yu I. Kostyukevich, A. S. Kononikhin, I. A. Popov, A. E. Bugrova, N. L. Starodubtseva, E. N. Nikolaev

    Research output: Contribution to journalArticlepeer-review

    Abstract

    At present, the secondary structure of oligonucleotide ions in a gas phase is almost not understood. One of the main points is the retention of a hairpin secondary structure during ionization. In this work, we used a deuterium–hydrogen exchange reaction in a gas phase at atmospheric pressure for studying the conformational dynamics of oligonucleotide ions formed as a result of electrospray ionization. The exchange reactions of two oligonucleotides, which consisted of the identical sets of nucleotides but differed in their sequence, have been studied. One of these oligonucleotides formed a hairpin secondary structure, but the other did not. It has been found that both of the oligonucleotides demonstrate similar reaction dynamics of deuterium/hydrogen exchange in the gas phase; thereby indicating that the secondary structure has been completely destroyed during ionization.

    Original languageEnglish
    Pages (from-to)427-432
    Number of pages6
    JournalHigh Energy Chemistry
    Volume50
    Issue number6
    DOIs
    Publication statusPublished - 1 Nov 2016

    Keywords

    • deuterium–hydrogen exchange
    • electrospray ionization
    • mass spectrometry
    • oligonucleotides
    • secondary structure

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