Application of a fast sorting algorithm to the assignment of mass spectrometric cross-linking data

Evgeniy V. Petrotchenko, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Cross-linking combined with MS involves enzymatic digestion of cross-linked proteins and identifying cross-linked peptides. Assignment of cross-linked peptide masses requires a search of all possible binary combinations of peptides from the cross-linked proteins' sequences, which becomes impractical with increasing complexity of the protein system and/or if digestion enzyme specificity is relaxed. Here, we describe the application of a fast sorting algorithm to search large sequence databases for cross-linked peptide assignments based on mass. This same algorithm has been used previously for assigning disulfide-bridged peptides (Choi et al., ), but has not previously been applied to cross-linking studies.

Original languageEnglish
Pages (from-to)1987-1989
Number of pages3
JournalProteomics
Volume14
Issue number17-18
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Bioinformatics
  • Crosslinking
  • Peptides
  • Protein complexes
  • Proteins

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