Antioxidant coating of micronsize droplets for prevention of lipid peroxidation in oil-in-water emulsion

Maria V. Lomova, Gleb B. Sukhorukov, Maria N. Antipina

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

Fast lipid peroxidation in emulsified oils results in carcinogens formation and product rancidity. Prevention of oxidative degradation in oil-in-water emulsion has been achieved by encapsulating of each droplet of dispersed phase in antioxidant multilayer coating shell. The fabrication comprised placing a surface-active ionic emulsifier at the oil/water interface followed by stepwise alternate adsorption a biocompatible polyelectrolyte and antioxidant layers. Uncoupled polyelectrolyte macromolecules and antioxidant were thoroughly removed from formulation, thus the protection was entirely attributed to the droplets' shell. The experiments were performed using linseed oil, the richest source of highly unstable omega-3 alpha linolenic essential fatty acid. Bovine serum albumin (BSA) was exploited as an anionic emulsifier. The biodegradable coating shell was formed of poly-l-arginine (PARG) and dextran sulfate (DS) applied as a polycation and a polyanion respectively. Tannic acid (TA) known as a natural antioxidant and possessing antimicrobial properties was used as a protective remedy. Oil microdroplets coated with TA-containing shell displayed physical-chemical and mechanical stability in aqueous phase and over freeze-drying process as determined by Χ-potential measurements, dynamic light scattering (DLS), and confocal laser scanning microscopy (CLSM). Oxidation of emulsified oil was monitored by formation of malondialdehyde (MDA) in the samples quantified by Thiobarbituric Acid Reactive Substances (TBARS) assay. Coating shell with an incorporated layer of TA effectively suppressed oxidation in water-dispersed oil droplets and affected iron-catalyzed oxidation over 15 days of incubation at 37 °C in 0.3 mM FeBr2 solution. Antioxidant activity of TA-containing shell assembled around each oil droplet was found to be higher than that of mixed tocopherols (MT) added to linseed oil in concentration of 10 000 ppm.

Original languageEnglish
Pages (from-to)3669-3676
Number of pages8
JournalACS Applied Materials and Interfaces
Volume2
Issue number12
DOIs
Publication statusPublished - 22 Dec 2010
Externally publishedYes

Keywords

  • Coating
  • Encapsulation
  • Layer-by-layer
  • Lipid peroxidation
  • Oil-in-water emulsion
  • Oxidative stability
  • Tannic acid

Fingerprint

Dive into the research topics of 'Antioxidant coating of micronsize droplets for prevention of lipid peroxidation in oil-in-water emulsion'. Together they form a unique fingerprint.

Cite this