Analytical potential of the in-electrospray ionization source hydrogen/deuterium exchange for the investigation of oligonucleotides

Yury Kostyukevich, Alexey Kononikhin, Igor Popov, Natalia Starodubtzeva, Stanislav Pekov, Eugene Kukaev, Maria Indeykinab, Eugene Nikolaev

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    It has previously been reported that different conformations of oligonucleotides may be detected using a gas-phase hydrogen/deuterium (H/D) exchange performed in the collision cell of a mass spectrometer. The presence of different conformers was postulated based on the bimodal shape of the deuterium distribution and on the ion mobility spectrometry data. Here we implement an in-electrospray ionization source H/D exchange to detect the different conformations of oligonucleotides in the region of ion formation. We observed that the number of H/D exchanges depends considerably on the temperature of the desolvating capillary and varies from 25% at 50°C to 80% at 450°C, but no bimodality in the shape of the deuterium distribution was observed. Such results indicate that in the region of ion formation different conformations of oligonucleotide ions rapidly interconvert one into another.

    Original languageEnglish
    Pages (from-to)59-63
    Number of pages5
    JournalEuropean Journal of Mass Spectrometry
    Volume21
    Issue number1
    DOIs
    Publication statusPublished - 2015

    Keywords

    • ESI
    • FT ICR
    • H/D exchange
    • Mass spectrometry
    • Oligonucleotides

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