Analysis of16 O/18 O and H/D Exchange Reactions between Carbohydrates and Heavy Water Using High-Resolution Mass Spectrometry

Lidiia Rumiantseva, Sergey Osipenko, Artem Zharikov, Albert Kireev, Evgeny N. Nikolaev, Yury Kostyukevich

Research output: Contribution to journalArticlepeer-review

Abstract

Mono-and polysaccharides are an essential part of every biological system. Identifying underivatized carbohydrates using mass spectrometry is still a challenge because carbohydrates have a low capacity for ionization. Normally, the intensities of protonated carbohydrates are relatively low, and in order to increase the corresponding peak height, researchers add Na+, K+, or NH4+ to the solution. However, the fragmentation spectra of the corresponding ions are very poor. Based on this, reliably identifying carbohydrates in complex natural and biological objects can benefit frommeasuring additional molecular descriptors, especially those directly connected to the molecular structure. Previously, we reported that the application of the isotope exchange approach (H/D and16 O/18 O) to high-resolution mass spectrometry can increase the reliability of identifying drug-like compounds. Carbohydrates possess many –OH and –COOH groups, making it reasonable to expect that the isotope exchange approach would have considerable potential for detecting carbohydrates. Here, we used a collection of standard carbohydrates to investigate the isotope exchange reaction (H/D and16 O/18 O) in carbohydrates and estimate its analytical applications.

Original languageEnglish
Article number3585
JournalInternational Journal of Molecular Sciences
Volume23
Issue number7
DOIs
Publication statusPublished - 1 Apr 2022

Keywords

  • O/Oexchange reaction
  • Carbohydrates
  • H/Dexchange
  • Mass spectrometry

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