Analysis of protein structure by cross-linking combined with mass spectrometry

Evgeniy V. Petrotchenko, Karl A.T. Makepeace, Jason J. Serpa, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Cross-linking combined with mass spectrometry is a powerful technique to study protein structure. Here, we present an optimized protocol for the preparation, processing, and analysis of a protein sample cross- linked with isotopically coded, affinity-enrichable, and CID-cleavable cross-linker CyanurBiotinDimercapto PropionylSuccinimide using LC/ESI-MS/MS on a Thermo Scientific Orbitrap mass spectrometer.

Original languageEnglish
Pages (from-to)447-463
Number of pages17
JournalMethods in Molecular Biology
Volume1156
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Chemical cross-linking
  • Isotope-labeled
  • Structural proteomics

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