Analysis of protein ratios based on the studies of human urine proteome in an experiment with 105-day isolation

L. Kh Pastushkova, O. A. Valeeva, A. S. Kononikhin, E. N. Nikolaev, I. A. Popov, I. M. Larina, I. V. Dobrokhotov, V. A. Ivanisenko, E. S. Tiys, N. A. Kolchanov

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose of the work was to study urine proteome of healthy human subjects during 105-day isolation in controlled environment of the IBMP chamber with a self-sustained life support system. The parameters under study were diurnal rhythm, physical activity, water intake, as well as consumption of sodium, protein and some other basic nutrients. Urine was sampled by 6 male subjects at the age of 25 to 40 years admitted to the experiment by the medical certification commission. The studies were performed with the use of proteome data acquisition technologies; the cutting-edge bio-information analysis techniques including reconstruction of associative gene networks were applied to investigate the urine proteome profile, to structure experimental data in light of the existing physiological concepts and to formulate viable hypotheses for ensuing experimental verification. Proteins of different origin were identified in urine; appearance or disappearance of proteins was in tight correlation with salt consumption.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalAviakosmicheskaya i Ekologicheskaya Meditsina
Volume46
Issue number2
Publication statusPublished - 2012
Externally publishedYes

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