Analysis of Expression of the Genes Containing LeR-1 and VeR-1 Sequences during Embryogenesis and Regeneration and in Intact Tissues of Newts

Yu V. Markitantova, K. A. Lukyanov, O. V. Kazanskaya, V. I. Mitashov, S. A. Lukyanov

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A method of construction of amplified cDNA libraries was developed on the basis of selective inhibition of cDNA amplification and modified for the studied models for analysis of expression of the genes containing LeR-1 and VeR-1 sequences. Time-related changes in expression of these genes were studied during regeneration of the adult lens and during embryogenesis of newts. The pattern of expression of the LeR-1 and VeR-1 genes proved to be not tissue-specific. A fragment adjoining 5′-area of the LeR-1 gene was obtained using a new approach: rapid amplification of cDNA ends by polymerase chain reaction (5′-RACE-PCR). Analysis of the LeR-1 clone primary structure using Gene Bank did not show essential homology with the known nucleotide sequences. Sequence LeR-1 is characterized by evolutionary conservatism of genomic DNA in Pleurodeles waltl, Xenopus laevis, and Rana temporaria.

Original languageEnglish
Pages (from-to)269-270
Number of pages2
JournalOntogenez
Volume28
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Amplified cDNA libraries
  • cDNA fragment
  • Embryogenesis
  • Expression
  • Lens and retina regeneration

Fingerprint

Dive into the research topics of 'Analysis of Expression of the Genes Containing LeR-1 and VeR-1 Sequences during Embryogenesis and Regeneration and in Intact Tissues of Newts'. Together they form a unique fingerprint.

Cite this