Ambient molecular imaging of dry fungus surface by electrospray laser desorption ionization mass spectrometry

Min Zong Huang, Sy Chyi Cheng, Siou Sian Jhang, Chih Chiang Chou, Chu Nian Cheng, Jentaie Shiea, Igor A. Popov, Eugene N. Nikolaev

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Ambient molecular imaging by Electrospray Laser Desorption/Ionization Mass Spectrometry (ELDI/MS) is described. The sample is sliced and placed onto the sample plate without further pretreatment. The distinctive distribution of the predominant chemical compounds on the surface of two dry fungi (Ganoderma lucidum and Antrodia camphorata) was obtained. Molecular images of hard and thick fungus slices were obtained for the first time, and both volatile and nonvolatile compounds originated from fungi were detected. The effects of the pulse laser frequency and the speed of the movement of the sample stage on the image quality were also investigated.

Original languageEnglish
Pages (from-to)172-182
Number of pages11
JournalInternational Journal of Mass Spectrometry
Volume325-327
DOIs
Publication statusPublished - 1 Jul 2012
Externally publishedYes

Keywords

  • Ambient
  • Electrospray ionization
  • Fungus
  • Imaging mass spectrometry
  • Laser desorption

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