Adaptive silver films for detection of antibody-antigen binding

Vladimir P. Drachev, Vishal C. Nashine, Mark D. Thoreson, Dor Ben-Amotz, V. Jo Davisson, Vladimir M. Shalaev

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)


Antibody-antigen binding events at a monolayer protein concentration have been demonstrated on nanostructured adaptive silver films (ASFs) using surface-enhanced Raman scattering (SERS) and luminescence-based assays. It is shown that proteins stabilize and restructure the ASF to increase the SERS signal while preserving antigen-binding activity. Evidence for antibody-antigen binding on the ASF substrates is the distinct SERS spectral changes of the surface-bound antibody or antigen without special tags. The activity of the surface-bound proteins and their practical application are validated by independent immunochemical assays. Results are presented to demonstrate that these surfaces can be extended to protein arrays with detection applications distinct from current SERS, fluorescence, or luminescence methods.

Original languageEnglish
Pages (from-to)8368-8373
Number of pages6
Issue number18
Publication statusPublished - 30 Aug 2005
Externally publishedYes


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