Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns

Evert Jan Sneekes, Jun Han, Monica Elliot, Juan Ausio, Remco Swart, Albert J.R. Heck, Christoph Borchers

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Due to their large diversity with respect to post-translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in-depth analysis of histones. In this paper, we present a separation strategy for histones based on free-flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS-DVB columns. The capillary columns are directly interfaced with an FT-ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.

Original languageEnglish
Pages (from-to)2691-2698
Number of pages8
JournalJournal of Separation Science
Volume32
Issue number15-16
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Accurate molecular weight analysis of histones
  • Capillary monolithic columns
  • Fourier transform ICR MS
  • Free flow electrophoresis

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