A study of the photoinduced conformational mobility of spin-labeled regenerated rhodopsin by ESR spectroscopy

P. V. Shelyakin, A. L. Kovarskii, V. V. Kasparov, T. B. Fel'dman, M. A. Ostrovskii

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Saturation transfer EPR spectroscopy is used to study the photoinduced conformational changes of spin-labeled (Cys140 and Cys316) rhodopsin in a photoreceptor membrane. Illumination of rhodopsin with "yellow" light (λ > 450 nm), converting it from the dark form into metarhodopsin II, a physiologically active form, is accompanied by an increase in the mobility of the cytoplasmic loop (Cys140) and eighth alpha-helix (Cys316), whereas the subsequent illumination with "blue" light (λ < 445 nm), which converts metarhodopsin II into a mixture of inactive photoregenerated products, results in a decrease in the mobility. The restoration of the specific features of the photoinduced changes in the conformational mobility of the cytoplasmic loops of rhodopsin regenerated in the dark is demonstrated.

Original languageEnglish
Pages (from-to)694-698
Number of pages5
JournalRussian Journal of Physical Chemistry B
Volume6
Issue number6
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

Keywords

  • EPR spectroscopy of spin labels
  • Photoinduced conformational changes
  • Rhodopsin

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