A standardized kit for automated quantitative assessment of candidate protein biomarkers in human plasma

Andrew J. Percy, Yassene Mohammed, Juncong Yang, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Background: An increasingly popular mass spectrometry-based quantitative approach for health-related research in the biomedical field involves the use of stable isotope-labeled standards (SIS) and multiple/selected reaction monitoring (MRM/SRM). To improve inter-laboratory precision and enable more widespread use of this 'absolute' quantitative technique in disease-biomarker assessment studies, methods must be standardized. Results/methodology: Using this MRM-with-SIS-peptide approach, we developed an automated method (encompassing sample preparation, processing and analysis) for quantifying 76 candidate protein markers (spanning >4 orders of magnitude in concentration) in neat human plasma. Discussion/conclusion: The assembled biomarker assessment kit - the 'BAK-76' - contains the essential materials (SIS mixes), methods (for acquisition and analysis), and tools (Qualis-SIS software) for performing biomarker discovery or verification studies in a rapid and standardized manner.

Original languageEnglish
Pages (from-to)2991-3004
Number of pages14
JournalBioanalysis
Volume7
Issue number23
DOIs
Publication statusPublished - Dec 2015
Externally publishedYes

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