A new method for normalization of the peptide retention times in chromatographic/mass-spectrometric experiments

D. M. Avtonomov, I. A. Agron, A. S. Kononikhin, I. A. Popov, E. N. Nikolaev

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Proteomic studies with the use of mass spectrometry required comparison of different experimenttal data (for example, control with a pathology or labeled and unlabeled samples). Identification of chromatographic peaks of the same substance in different chromatograms (time normalization of chromatograms) is complicated if peptides are identified in different experiments according only to their exactly evaluated masses and retention times on a chromatographic column. Retention times of the same peptides would vary from one experiment to another due to inevitable differences in experimental chromatographic conditions (replacement of chromatographic columns, slight changes in flow rates of a mobile phase or in a solvent concentration, or in other conditions). We proposed a reliable method for selection of peaks that corre sponded to the same peptides from chromatography/mass spectra for the subsequent alignment of retention times (both a linear and some other monotone function).

Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalRussian Journal of Bioorganic Chemistry
Volume37
Issue number2
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

Keywords

  • Alignment of retention times
  • Chromatography
  • Mass spectrometry
  • Method of accurate mass and time tag
  • Proteomics

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