6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype

Daria Elkina, Lennart Weber, Marcus Lechner, Olga Burenina, Andrea Weisert, Elena Kubareva, Roland K. Hartmann, Gabriele Klug

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic α-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12–16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA ≥ 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the ΔssrS strain when grown in the presence of 250 mM NaCl.

Original languageEnglish
Pages (from-to)1627-1637
Number of pages11
JournalRNA Biology
Volume14
Issue number11
DOIs
Publication statusPublished - 2 Nov 2017
Externally publishedYes

Keywords

  • (photo)oxidative stress
  • 6S RNA
  • high salt stress
  • Northern blot analysis
  • pRNA synthesis
  • RNA-Seq
  • ssrS deletion

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