5S rRNA sugar-phosphate backbone protection in complexes with specific ribosomal proteins

Olga V. Shpanchenko, Maria I. Zvereva, Olga A. Dontsova, Knud H. Nierhaus, Alexey A. Bogdanov

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

5S ribosomal RNA forms stable specific complexes with ribosomal proteins L18, L25 and L5. In this work, interaction of phosphate residues of E. coli 5S rRNA within 5S rRNA-protein complexes has been studied. For this purpose 5S rRNA with statistically distributed phosphorothioate residues has been used for complex formation and the accessibility of phosphorothioates to iodine cleavage in the complex and in the free state has been studied. In free 5S rRNA, the phosphate residue at A73 was partially protected, probably due to being involved in the organization of the spatial structure of 5S rRNA. This protection is stronger in the complex with three proteins when the 5S rRNA structure is stabilized. In the 5S rRNA-L18 complex only two phosphate groups, G7 and A34, were protected. L25 in a complex with 5S rRNA protects large numbers of phosphorothioate groups concentrating in two clusters, indicating the possibility of two binding sites for this protein on 5S rRNA. The protection pattern differs from that for individual proteins because of the possible rearrangement of the structure.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalFEBS Letters
Volume394
Issue number1
DOIs
Publication statusPublished - 23 Sep 1996
Externally publishedYes

Keywords

  • 5S Ribosomal RNA
  • Footprinting
  • Iodine cleavage
  • Phosphorothioate
  • Ribosomal protein
  • RNA structure
  • RNA-protein interaction

Fingerprint

Dive into the research topics of '5S rRNA sugar-phosphate backbone protection in complexes with specific ribosomal proteins'. Together they form a unique fingerprint.

Cite this