АНАЛИЗ ИЗМЕНЕНИЙ СОСТАВА ФОСФАТИДИЛХОЛИНОВ В ГЛИОБЛАСТОМАХ ЧЕЛОВЕКА EX VIVO

Translated title of the contribution: Analysis of phosphatidylcholines alterations in human glioblastoma multiform tissues ex vivo

S. I. Pekov, A. A. Sorokin, A. A. Kuzin, K. V. Bocharov, D. S. Bormotov, A. S. Shivalin, V. A. Shurkhay, A. A. Potapov, E. N. Nikolaev, I. A. Popov

Research output: Contribution to journalArticlepeer-review

Abstract

Significant metabolism alteration is accompanying the cell malignization process. Energy metabolism disturbance leads to the activation of de novo synthesis and beta-oxidation processes of lipids and fatty acids in a cancer cell, which becomes an indicator of pathological processes inside the cell. The majority of studies dealing with lipid metabolism alterations in glial tumors are performed using the cell lines in vitro or animal models. However, such conditions do not entirely represent the physiological conditions of cell growth or possible cells natural variability. This work presents the results of the data obtained by applying ambient mass spectrometry to human glioblastoma multiform tissues. By analyzing a relatively large cohort of primary and secondary glioblastoma samples, we identify the alterations in cells lipid composition, which accompanied the development of grade IV brain tumors. We demonstrate that primary glioblastomas, as well as ones developed from astrocytomas, are enriched with mono- and diunsaturated phosphatidylcholines (PC 26:1, 30:2, 32:1, 32:2, 34:1, 34:2). Simultaneously, the saturated and polyunsaturated phosphatidylcholines and phosphatidylethanolamines decrease. These alterations are obviously linked to the availability of the polyunsaturated fatty acids and activation of the de novo lipid synthesis and beta-oxidation pathways under the anaerobic conditions in the tumor core.

Translated title of the contributionAnalysis of phosphatidylcholines alterations in human glioblastoma multiform tissues ex vivo
Original languageRussian
Pages (from-to)81-87
Number of pages7
JournalBiomeditsinskaya Khimiya
Volume67
Issue number1
DOIs
Publication statusPublished - 2021

Fingerprint

Dive into the research topics of 'Analysis of phosphatidylcholines alterations in human glioblastoma multiform tissues ex vivo'. Together they form a unique fingerprint.

Cite this